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Protein Patterns and Plasmid Profiles of the Bacterial Strains Isolated from a Poultry Slaughterhouse in Ankara, Turkey

Leyla Açık1*, Ayhan Temiz2, Ayten Çelebi3, Sevim Arslan1 and Remziye Yılmaz2


1
Gazi Üniversitesi, Fen Edebiyat Fakültesi, Biyoloji Bölümü, Teknikokullar, Ankara, Turkey

2Hacettepe Üniversitesi, Gıda Mühendislighi Bölümü, Ankara, Turkey
3Kırıkkale Üniversitesi, Biyoloji Bölümü, Kırıkkale, Turkey

Article history:

Received November 1, 2004
Accepted June 28, 2005

Key words:

poultry, bacteria, SDS-PAGE, plasmid

Summary:

A total of 65 identified isolates of coliform bacteria Salmonella, including Campylobacter and Staphylococcus isolated from different control points of a poultry slaughterhouse in Ankara, Turkey were characterized by morphological, biochemical and physiological tests including API 10 S system, and by plasmid profiles on agarose gel electrophoresis and whole-cell protein patterns on SDS-PAGE. Plasmids were detected in 53.8 % of the isolates. The molecular mass of the plasmids was within the range from 0.66 to 12.66 mDa. Electrophoretic banding patterns showed that whole cell protein profiles differed in several protein bands in Salmonella, Campylobacter and Staphylococcus species, but the differences were insufficient for reliable differentiation of bacteria species by SDS-PAGE method.



*Corresponding author:     leylaacik@gazi.edu.tr
                                                      ++(312) 21 26 030

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