Multibioreaction Methodology for Baeyer-Villiger Monooxygenase Monitoring

Regina A. C. Gonçalves^1,a, André L. M. Porto¹, Lucimar Pinheiro¹, José R. Cagnon¹, Gilson P. Manfio^2,b
and Anita J. Marsaioli¹*

¹Chemistry Institute, State University of Campinas, CP 6154, CEP 13083-970, Campinas (SP), Brazil
^aPresent address: State University of Maringa (UEM), DFF, CEP 87020-900, Maringá (PR), Brazil
²Fundação Tropical »André Tosello«, Rua Latino Coelho 1301, CEP 13087-010, Campinas (SP), Brazil
^bPresent address: CPQBA, State University of Campinas, CP 6171, CEP 13083-970, Campinas (SP), Brazil

Article history:
Received July 26, 2004
Accepted November 22, 2004

Key words:
Baeyer-Villiger monooxygenase, alkene monooxygenase, multibioreaction

Summary:
Baeyer-Villiger monooxygenase (BVMO) activity was monitored using traditional biocatalytic methods and also using a multibioreaction approach. The prochiral ketones 4-methyl- cyclohexanone and 3-hexyl-cyclobutanone, among others, were used in screening for BVMO in several microorganisms, leading to the selection of Geotrichum candidum CCT 1205, Aspergillus oryzae CCT 0975, Curvularia lunata CCT 5629, Aspergillus niger CCT 5559, Trichoderma sp. CCT 5551, Cunninghamella echinulata CCT 4424 and Cunninghamella echinulata CCT 4259 as good candidates for further BVMO investigations. Additionally, a multibioreaction methodology was used to confirm the presence of BVMO, an activity previously detected by a rapid fluorescence methodology. It was therefore possible to confirm the presence of a BVMO, more precisely a cyclohexanone monooxygenase (CHMO) and also to reveal the presence of an alkene monooxygenase in Trichosporum cutaneum CCT 1903.

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