Differentiation Between Amino Acids Used as Carbon and Energy Sources During Growth of Geotrichum candidum Geo17
Lydia Adour2, Catherine Couriol1 and Abdeltif Amrane1*
1LARCIP/LPS, Université de Rennes 1, Campus de Beaulieu, Bât. 10 A, CS 74205, Avenue du Général Leclerc, FR-35042 Rennes Cedex, France
2Laboratoire de Chimie Appliquée et de Génie Chimique, Université Mouloud Mammeri, Hasnaoua 1, Tizi-Ouzou, Algérie
Article history:
Received July 27, 2004
Accepted November 22, 2004
Key words:
amino acids, carbon and nitrogen sources, Geotrichum candidum, growth
Summary:
Geotrichum candidum Geo17 was cultivated on peptones as carbon and nitrogen source, and in the presence of lactate as the second carbon source. From the analysis of the initial and final culture medium after total hydrolysis, the yield of consumption was determined for each amino acid. Amino acids have been considered a convenient carbon source for biosynthesis, while the rest of the amino acids were assumed to be used only as a nitrogen source, with the corresponding carbon released as CO2 resulting from energy supply. Carbon mass balances confirmed this assumption. A clear differentiation between the amino acids assimilated as carbon sources and those assimilated as energy sources was therefore highlighted.
*Corresponding author:
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