Lentinula edodes Biotechnology – From Lentinan to Lectins

Valentina E. Nikitina1, Olga M. Tsivileva1*, Alexei N. Pankratov2 and Nikolai A. Bychkov1

Laboratory of Microbiology and Mycology, Institute of Biochemistry and Physiology of Plants and Microorganisms, Russian Academy of Sciences, 13 Entuziastov Avenue, 410049 Saratov, Russia

2Department of Chemistry, N. G. Chernyshevskii Saratov State University, 83 Astrakhanskaya Street, 410012 Saratov, Russia

Article history:

Received September 27, 2006
Accepted May 18, 2007

Key words:

submerged culture, Lentinula, lectins of higher fungi, brown mycelial film, molecular structure, quantum chemical study


Lentinula edodes was the first medicinal macrofungus to enter the realm of modern biotechnology. The present paper briefly reviews the history of the modern biotechnology of this mushroom starting with the production of the polysaccharide preparation lentinan, and ending with an overview of our own work regarding the production of lectins. Our work with lectins has involved studies of the effect of initial pH, carbon and nitrogen sources and the C:N ratio on lectin production in both the mycelium and culture medium. We have shown that lectin activity is related to morphological development, with the activity being highest in extracts of the pigmented mycelial films that precede fruiting body production.


*Corresponding author:           This email address is being protected from spambots. You need JavaScript enabled to view it.
                                               ++7 8452 970 444
                                               ++7 8452 970 383