In vitro Antioxidant, Cytotoxic and Antidiabetic Activity of Protein Hydrolysates Prepared from Chinese Pond Turtle (Chinemys reevesii)
Md. Serajul Islam1,2, Wang Hongxin1,2*, Habtamu Admassu3, Amer Ali Mahdi1,2, Ma Chaoyang2 and Fu An Wei4
1State Key Laboratory of Food Science and Technology, Jiangnan University, 1800 Lihu Avenue, Wuxi-214122, Jiangsu province, PR China
2National Engineering Research Center for Functional Food, Jiangnan University, 1800 Lihu Avenue, Wuxi-214122, Jiangsu province, PR China
3Biotechnology and Bioprocessing Center of Excellence/Department of Food Process Engineering, College of Biological and Chemical Engineering, Addis Ababa Science and Technology University, Addis Ababa, Ethiopia
4Guangxi Zhongtaikang Technology Industry Co., Ltd., Nanning-530029, Guangxi, PR China
Received: 1 December 2020
Accepted: 15 July 2021
Chinese pond turtle, molecular mass profiles, antioxidant activity, antidiabetic capacity, lipid peroxide inhibition, cytotoxic effect
Research background. Cardiovascular diseases and diabetes are the biggest causes of death globally. Bioactive peptides derived from many food proteins using enzymatic proteolysis and food processing have a positive impact on the prevention of these diseases. The bioactivity of Chinese pond turtle muscle proteins and their enzymatic hydrolysates has not received much attention, thus this study aims to investigate their antioxidant, antidiabetic and cytotoxic activities.
Experimental approach. Chinese pond turtle muscles were hydrolysed using four proteolytic enzymes (Alcalase, Flavourzyme, trypsin and bromelain) and the degrees of hydrolysis were measured. High-performance liquid chromatography (HPLC) was conducted to explore the amino acid profiles and molecular mass distribution of the hydrolysates. The antioxidant activities were evaluated using various in vitro tests, including 1,1-diphenyl-2-picrylhydrazyl (DPPH) and 2,2’-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS), hydroxyl radical scavenging activity, reducing capacity, chelating Fe2+ and lipid peroxide inhibition activity. Antidiabetic activity was evaluated using α-amylase inhibition and α-glucosidase inhibition assays. Besides, cytotoxic effect of hydrolysates on human colon cancer (HT-29) cells was assessed.
Results and conclusions. The amino acid composition of the hydrolysates revealed higher mass fractions of glutamic, aspartic, lysine, hydroxyproline and hydrophobic amino acids. Significantly highest inhibition of lipid peroxidation was achieved when hydrolysate obtained with Alcalase was used. Protein hydrolysate produced with Flavourzyme had the highest radical scavenging activity measured by DPPH (68.32 %), ABTS (74.12 %) and FRAP (A700 nm=0.300) assays, α-glucosidase (61.80 %) inhibition and cytotoxic effect (82.26 %) on HT-29 cell line at 550 μg/mL. Hydrolysates obtained with trypsin and bromelain had significantly highest (p<0.05) hydroxyl radical scavenging (92.70 %) and Fe2+ metal chelating (63.29 %) activities, respectively. The highest α-amylase (76.89 %) inhibition was recorded when using hydrolysates obtained with bromelain and Flavourzyme.
Novelty and scientific contribution. Enzymatic hydrolysates of Chinese pond turtle muscle protein had high antioxidant, cytotoxic and antidiabetic activities. The findings of this study indicated that the bioactive hydrolysates or peptides from Chinese pond turtle muscle protein can be potential ingredients in pharmaceuticals and functional food formulations.