A Fast Immunoradiometric Assay of Human Growth Hormone
Boris Mildner*, Živko Gnjidić
University hospital »Sestre milosrdnice«, Vinogradska c. 29, 10000 Zagreb, Croatia
Received February 27, 1997
Accepted March 21, 1997
human growth hormone, acromegaly, pituitary adenoma, RIA, TRIVIA
Using the components of "Pharmacia" two-site immunoradiometric assay (IRMA) it is possible to determine human growth hormone (hGH) in serum in the time interval of approximately 2 h if the reaction is performed at 37 °C (modified IRMA). The standard IRMA, that is the assay performed at room temperature as recommended by the manufacturer last at least 4 h. Comparison of the results obtained by the standard "Pharmacia" protocol, i.e. modified IRMA vs. standard IRMA, yielded a correlation coefficient of r = 0.9996. The results of hGH determinations obtained by two IRMA assays were also compared with the results obtained by standard competitive radioimmunoassay (RIA) of hGH. The following correlation coefficients were obtained: RIA vs. standard IRMA r = 0.9997; RIA vs. modified IRMA r = 0.9989. The modified IRMA procedure has found its value in determination of hGH in serum during and immediately after the selective removal of the pituitary (somatotroph) adenoma.
*Corresponding author: present adress PLIVA dd, Research Institute, Department of Biosynthesis and Biotechnology, Prilaz baruna Filipovića 25, 10000 Zagreb, Croatia