Alpha Amylase Production by Bacillus cereus MTCC 1305 Using Solid-State Fermentation

Hema Anto, Ujjval Trivedi and Kamlesh Patel*

Department of Biosciences, Sardar Patel University, Vallabh Vidyanagar 388 120, Gujarat, India

Article history:

Received October 31, 2005

Accepted February 28, 2006

Key words:

α-amylase, Bacillus cereus, solid-state fermentation, wheat bran, optimization


Production of α-amylase under solid-state fermentation by Bacillus cereus MTCC 1305 has been investigated using wheat bran and rice flake manufacturing waste as substrates. With wheat bran, highest enzyme production expressed as units per mass of dry substrate ((94±2) U/g) was observed. Production parameters were optimized as inoculum size 10 % (volume per mass) and substrate:moisture ratio 1:1. Among different carbon sources supplemented, glucose (0.04 g/g) showed enhanced enzyme production ((122±5) U/g). Supplementation of different nitrogen sources (0.02 g/g) showed decline in enzyme production. Optimum α-amylase enzyme activity was observed at 55 °C and pH=5. At 75 °C, enzyme showed 90 % activity compared to 55°C.

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