Formation of Edible Soybean and Soybean-Complex Protein Films by a Cross-Linking Treatment with a New Streptomyces Transglutaminase

Guocheng Su1, Huinong Cai1, Changyi Zhou1 and Zhang Wang2*

Bio-tech Engineering College, Jimei University, Xiamen 361021, Fujian, PR China

2China National Research Institute of Food and Fermentation Industries, Beijing 100027, PR China

Article history:

Received April 30, 2006
Accepted December 14, 2006

Key words:

transglutaminase, edible films, soybean protein isolates, whey protein isolates, β-lactoglobulin, mechanical properties, barrier properties


Soybean protein isolate (SPI) was used in the investigation of the formation of edible protein films through an enzymatic cross-linking method with a purified microbial transglutaminase (MTG) produced from a new effective strain Streptomyces sp. WZFF.L-M1 preserved in our laboratory, followed by the addition of glycerol and suitable heating and drying treatments. Cheaper partially-purified skimmed soybean protein powder (SSP) and whey protein isolates (WPI) were used as the substitutes partially replacing the expensive SPI products, and purified β-lactoglobulin was taken as the positive control of WPI. As a result, the three alternatives could also form highly efficient edible films under the optimal operation conditions. The films made with SPI alternatives, about 50 mm thin, had homogenous network structures, without any holes by direct observation with the naked eye. The tests of the properties of these films showed that they had high water-keeping capacity and strong elasticity, that the ultimate tensile strength (TS) and the elongation at break (Eb) had been remarkably increased (TS>5 MPa, Eb>50 %), and that the prevention rates against the permeability of water vapour and oxygen in the air were also upgraded more than 85 and 70 %, respectively.


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