Improvement of Xylanase Production by Cochliobolus sativus in Submerged Culture

Yasser Bakri, Mohammed Jawhar and Mohammed Imad Eddin Arabi*

Department of Molecular Biology and Biotechnology, AECS, P.O. Box 6091, SY-Damascus, Syria

Article history:
Received August 29, 2006
Accepted October 5, 2007

Key words:
xylanase, Cochliobolus sativus, waste utilization, submerged fermentation

Summary:
The xylanase production by a new Cochliobolus sativus Cs5 strain was improved under submerged fermentation. The xylanase was induced by xylan and repressed by glucose, sucrose, maltose, xylose, starch and cellulose. Highest enzyme production (98.25 IU/mL) was recorded when wheat straw (4 % by mass per volume) was used as a carbon source after 120 h of incubation. NaNO₃ increased xylanase production 5.4-fold as compared to the control. Optimum initial pH was found to be 4.5 to 5. The C. sativus Cs5 strain grown under submerged culture in a simple medium proved to be a promising microorganism for xylanase production.

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