https://doi.org/10.17113/ftb.64.03.26.9373

Article in press

Structural, Functional and Antioxidant Properties of Soy Protein Concentrates Enriched with Black Mulberry Pomace

Nevena Barać¹, Biljana Rabrenović¹, Ivana Sredović Ignjatović¹, Steva Lević¹, Vladimir Pavlović¹, Slađana Žilić², Danka Milovanović² and Miroljub Barać^1*

¹Faculty of Agriculture, University of Belgrade, Nemanjina 6, 11080 Belgrade, Serbia

²Maize Research Institute, Slobodana Bajića 1, Zemun polje, 11185 Belgrade, Serbia

Copyright © 2024 This is a Diamond Open Access article published under CC-BY licence. Copyright remains with the authors, who grant third parties the unrestricted right to use, copy, distribute and reproduce the article as long as the original author(s) and source are acknowledged.

Food Technol. Biotechnol. 2026; 64(3).

Article history:

Received: 16 September 2025

Accepted: 10 April 2026

Keywords:

soy protein concentrate; black mulberry pomace; polyphenol-protein interactions; antioxidant properties; techno-functional performance; sustainable co-ingredients

Summary:

Research background. The incorporation of fruit processing residues into protein-rich ingredients is gaining attention as a sustainable strategy to enhance the nutritional and functional value of plant-based formulations. However, the influence of black mulberry pomace addition on protein matrix structure, functionality, and digestibility remains insufficiently explored. This study investigates the potential of using black mulberry pomace as a co-ingredient to modify and improve the characteristics of soy protein concentrate derived from moderately toasted soy flour.

Experimental approach. Soy protein concentrates were obtained by washing soy flour with ethanol after blending it with increasing proportions of black mulberry pomace. The prepared samples were examined for their chemical composition, in vitro digestibility, hydration and emulsifying properties, and antioxidant potential using standard radical scavenging and metal-chelating assays. Structural characteristics were investigated using infrared spectroscopy and electron microscopy. Relationships among compositional, functional, and antioxidant parameters were explored using multivariate statistical analysis.

Results and conclusions. Black mulberry pomace addition improved hydration and emulsifying properties at lower inclusion levels, while enhancing antioxidant potential through phenolic incorporation. These enhancements were attributed to interactions between phenolic compounds and protein structures, confirmed by spectroscopic and microscopic evidence. However, higher pomace concentrations negatively affected protein digestibility, likely due to matrix densification and aggregation. Correlation and multivariate analyses confirmed close associations between antioxidant activity, protein solubility, and techno-functional performance. These findings suggest that low to moderate pomace levels are sufficient to induce positive changes without compromising digestibility.

Novelty and scientific contribution. This work provides new insights into the dual role of black mulberry pomace as a functional and antioxidant-enhancing agent in plant protein systems. It highlights a sustainable approach for the development of value-added protein ingredients by integrating underutilised agri-food residues. The comprehensive combination of chemical, functional, and structural analysis presented in this study contributes to a better understanding of how co-ingredient interactions can shape the bio-functional performance of soy-based matrices.