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Effect of Ripening in Brine and in a Vacuum on Protein, Fatty Acid and Mineral Profiles, and Antioxidant Potential of Reduced-Fat White Cheese

Miroljub Barać1*orcid tiny, Zlatan Sarić2orcid tiny, Tanja Vučić1orcid tiny, Ivana Sredović Ignjatović1, Danijel Milinčić1orcid tiny, Bojana Špirović Trifunović1orcid tiny and Milenko Smiljanić3

1University of Belgrade, Faculty of Agriculture, Nemanjina 6, 11080 Zemun, Serbia

2University of Sarajevo, Faculty of Agriculture and Food Sciences, Zmaja od Bosne 8, 71000 Sarajevo, Bosnia and Herzegovina

3University of East Sarajevo, Faculty of Technology, Karakaj 34A, 75400 Zvornik, Bosnia and Herzegovina

Article history:

Received: 9 July 2020

Accepted: 10 February 2021

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Key words:

reduced-fat white cheese, proteolysis, fatty acids, mineral profile, antioxidant properties


Research backgroundNumerous factors affect the ripening of reduced-fat white cheese. The aim of this study is to investigate the influence of ripening environment (brine or vacuum plastic bags without brine) on the chemical composition, protein, fatty acid profile and mineral content as well as antioxidant properties of industrially produced reduced-fat white cheese. 

Experimental approach. A low-fat white cheese was manufactured on an industrial scale from milk that remained after the production of kajmak and ripened for 60 days at 4 °C after packaging in a polystyrene container with brine containing 6 % salt or in vacuum-sealed polyethylene bags. The influence of ripening environment on proteolysis was monitored by the change of soluble nitrogen fractions as well as by sodium dodecyl sulphate–polyacrylamide gel electrophoresis of Tris(hydroxymethyl) aminomethane-HCl extracts of cheese proteins under non-reducing conditions and water-soluble fractions under reducing conditions. An effect that ripening environment had on fatty acid and mineral content was also monitored. The change of antioxidant potential of the investigated cheese during ripening led to the change of iron(II) chelating ability, reducing power and free-radical scavenging activity. 

Results and conclusions. The ripening environment differently affected proteolysis, fatty acid composition, mineral profiles and antioxidant properties of reduced-fat white cheese. White cheese ripened in brine had more intensive proteolytic changes than the cheese ripened in a vacuum, but also more intensive diffusion processes, especially between the 40th and 60th day of ripening. The brine-ripened cheese had higher values of water-soluble nitrogen content, but lower contents of trichloroacetic acid-soluble and phosphotungstic acid-soluble nitrogen than a vacuum ripened cheese. Cheese ripened in brine had a lower content of almost all investigated macro- and microelements. After 60 days of ripening, in the cheese ripened in brine only myristic (C14:0) and palmitic acid (C16:0) were detected, whereas in a vacuum-ripened cheese C10:0-C16:0 fatty acids dominated. Vacuum-ripened reduced-fat cheese had more favourable reducing power, while white brined reduced-fat cheese had better radical scavenging activity and iron(II) chelating activity.

Novelty and scientific contribution. These results suggest significant influence of ripening conditions (immersion in brine or in vacuum-sealed polyethylene bags) on nutritive and functional properties of reduced-fat white cheese. Ripening in a vacuum has become a useful method for obtaining high-value reduced-fat white cheese.

*Corresponding author: +381114413268

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