A Fast Immunoradiometric Assay of Human Growth Hormone

Boris Mildner*, Živko Gnjidić

University hospital »Sestre milosrdnice«, Vinogradska c. 29, 10000 Zagreb, Croatia

Article history:
Received February 27, 1997
Accepted March 21, 1997

Key words:
human growth hormone, acromegaly, pituitary adenoma, RIA, TRIVIA

Summary:
Using the components of "Pharmacia" two-site immunoradiometric assay (IRMA) it is possible to determine human growth hormone (hGH) in serum in the time interval of approximately 2 h if the reaction is performed at 37 °C (modified IRMA). The standard IRMA, that is the assay performed at room temperature as recommended by the manufacturer last at least 4 h. Comparison of the results obtained by the standard "Pharmacia" protocol, i.e. modified IRMA vs. standard IRMA, yielded a correlation coefficient of r = 0.9996. The results of hGH determinations obtained by two IRMA assays were also compared with the results obtained by standard competitive radioimmunoassay (RIA) of hGH. The following correlation coefficients were obtained: RIA vs. standard IRMA r = 0.9997; RIA vs. modified IRMA r = 0.9989. The modified IRMA procedure has found its value in determination of hGH in serum during and immediately after the selective removal of the pituitary (somatotroph) adenoma. 

*Corresponding author: present adress PLIVA dd, Research Institute, Department of Biosynthesis and Biotechnology, Prilaz baruna Filipovića 25, 10000 Zagreb, Croatia