getpdf NLM PubMed Logo https://doi.org/10.17113/ftb.59.04.21.7076  

Increased Survival of Lactococcus lactis Strains Subjected to Freeze-Drying after Cultivation in an Acid Medium: Involvement of Membrane Fluidity

Aurore Bodzen1,2orcid tiny, Audrey Jossier1orcid tiny, S├ębastien Dupont1orcid tiny, Pierre-Yves Mousset2, Laurent Beney1orcid tiny, Sophie Lafay2 and Patrick Gervais1*orcid tiny

1University of Burgundy, AgroSup Dijon, PAM UMR A 02.102, F-21000 Dijon, France

2Indigo Therapeutics, 5 rue Salneuve, 75017 Paris, France

Article history:

Received: 26 November 2020

Accepted: 29 September 2021

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Key words:

freeze-drying, viability, membrane fluidity, acid prestress, Lactococcus lactis

Summary:

Research background. Freeze-drying is the most widely used dehydration process in the food industry for the stabilization of bacteria. Studies have shown the effectiveness of an acid prestress in increasing the resistance of lactic acid bacteria to freeze-drying. Adaptation of bacteria to an acid stress is based on maintaining the properties of the plasma membrane. Indeed, the fatty acid composition of the membrane of lactic acid bacteria is often changed after an acid prestress. However, few studies have measured membrane fluidity after an acid stress during lactic acid bacterial strain cultivation.

Experimental approach. In order to use two pH profiles, the strains Lactococcus lactis NCDO 712 and NZ9000 were cultivated in two media, without any pH control. The two pH profiles obtained were representative of the initial medium composition, medium buffering properties and strain metabolism. Absorbance at 600 nm and pH were measured during bacterial cultivation. Then, the two strains were freeze-dried and their survival rates determined. Membrane fluidity was evaluated by fluorescence anisotropy measurements using a spectrofluorometer. 

Results and conclusionsCultivation under more acidic conditions significantly increased the survival during freeze-drying (p<0.05, ANOVA) of both strains. Moreover, in both strains of L. lactis, a more acidic condition during cultivation significantly increased membrane fluidity (p<0.05, ANOVA). Our results revealed that cultivation under such conditions, fluidifies the membrane and allows a better survival during freeze-drying of the two L. lactis strains. A more fluid membrane can facilitate membrane deformation and lateral reorganization of membrane components, critical for the maintenance of cellular integrity during dehydration and rehydration.

Novelty and scientific contribution. A better understanding of the involvement of membrane properties, especially of membrane fluidity, in bacterial resistance to dehydration is provided in this study.

*Corresponding author: +33380774008
  patrick.gervais@u-bourgogne.fr

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