Glucose and Maltose Metabolism in MIG1-disrupted and MAL-constitutive Strains of Saccharomyces cerevisiae

Christopher J. L. Klein¹, Lisbeth Olsson¹*, Birgitte Ronnow², Jørn Dalgaard Mikkelsen², Jens Nielsen<sup>1

1</sup>Center for Process Biotechnology, Department of Biotechnology, Building 223,Technical University of Denmark, DK-2800 Lyngby, Denmark
²Danisco Biotechnology, PO Box 17, Langebrogade 1, DK-1001 Copenhagen K, Denmark

Article history:
Received September 10, 1997
Accepted October 21, 1997

Key words:
Saccharomyces cerevisiae, baker's yeast, glucose repression, catabolite inactivation, A/I1G1, MAL, genetic engineering

Summary:
The alleviation of glucose control of maltose metabolism brought about by MIG1 disruption was compared to that by MAL overexpression in a haploid Saccharomyces cerevisiae strain. The sugar consumption profiles during cultivation of the wild type, single transformants and a double transformant in a mixed glucose-maltose medium revealed that the MAL-constitutive strains zvere more alleviated than the single MIGl-disrupted transformant. While all transformants exhibited higher maximum specific growth rates (0,24-0.25 h^-1) in glucose-maltose mixtures than the wild type strain (0.20 h^-1), the MAL-constitutive transformants grew even faster (0.27-0.30 h^-1) in pure glucose medium than the wild type strain (0.24 h^-1).

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