Solid Culturing of Bacillus amyloliquefaciens for Alpha Amylase Production

Dhanya Gangadharan, Swetha Sivaramakrishnan, Kesavan Madhavan Nampoothiri and Ashok Pandey*

Biotechnology Division, Regional Research Laboratory, CSIR, Trivandrum 695019, India

Article history:

Received November 30, 2005
Accepted March 22, 2006

Key words:

alpha amylase, Bacillus amyloliquefaciens, solid-state fermentation, agroresidues


Fourteen different agroresidues were screened for alpha amylase production using Bacillus amyloliquefaciens ATCC 23842. Among them, wheat bran (WB) and groundnut oil cake (GOC) in mass ratio of 1:1 was proved as the best substrate source. Supplementation with 0.01 M KH2PO4 and 1 % soluble starch enhanced the enzyme yield considerably. Maximum enzyme recovery from the solid mass was obtained when extracted with 0.1 M acetate buffer, pH=5.0. Maximum enzyme titer expressed as units per mass of dry substrate obtained was 62 470 U/g after 72 hours of fermentation at 37 °C by using the above solid substrate mixture (5 g) with the initial moisture of 85 % and inoculated with Bacillus amyloliquefaciens of 2·109 CFU/mL.

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