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Nutritional Requirements for the Mycelial Biomass and Exopolymer Production by Hericium erinaceus CZ-2

Daming Huang1, Fengjie Cui1*, Yin Li2, Zhicai Zhang1, Jiewen Zhao1, Xiaoming Han1, Xiang Xiao1, Jingya Qian1, Qifei Wu1 and Guoqiang Guan1


1
Institute of Bioengineering, School of Food and Biological Technology, Jiangsu University, 212013 Zhenjiang, PR China

2Department of Plant Science, North Dakota State University, 58105 Fargo, USA

Article history:

Received August 26, 2006
Accepted March 2, 2007

Key words:

Hericium erinaceus, submerged culture, medium optimization, mycelial biomass, exopolymer, orthogonal array design method

Summary:

In this work, the effects of medium composition and fermentation parameters on the simultaneous production of mycelial biomass and exopolymer by medicinal mushroom Hericium erinaceus CZ-2 were investigated in shake flask cultures using one-factor-at-a-time method and orthogonal array design. Results showed that the most suitable carbon, nitrogen, mineral sources, and cofactors for the mycelial biomass and exopolymer production were: corn flour combined with 1 % glucose, yeast extract, KH2PO4 and corn steep liquor. The intuitive analysis of orthogonal array design results indicated that the effects of nutritional requirement on the mycelial growth of Hericium erinaceus CZ-2 were in regular sequence of corn flour combined with 1 % glucose > yeast extract > corn steep liquor > KH2PO4, and those on exopolymer production were in the order of corn flour combined with glucose > KH2PO4 > yeast extract > corn steep liquor. The maximal yield of mycelial biomass (16.07 g/L) was obtained when the composition of the culture medium was (in g/L): corn flour 30, glucose 10, yeast extract 3, KH2PO4 1, CaCO3 0.5, and 15 mL/L of corn steep liquor; while the maximal exopolymer yield (1.314 g/L) was achieved when the composition of medium was (in g/L): corn flour 30, glucose 10, yeast extract 5, KH2PO4 3, CaCO3 0.5, and 15 mL/L of corn steep liquor. In the 15-litre scale-up fermentation, the maximum mycelial biomass yield of 20.50 g/L was achieved using the optimized medium.

 


*Corresponding author:          fengjiecui@163.com
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