Fermentation of Shrimp Biowaste under Different Salt Concentrations with Amylolytic and Non-Amylolytic Lactobacillus Strains for Chitin Production

Mukku Shrinivas Rao* and Willem F. Stevens

Food Engineering and Bioprocess Technology, Asian Institute of Technology, PO Box 4, Klong Luang, Pathumthani 12120, Thailand

Article history:

Received June 26, 2005
Accepted October 3, 2005

Key words:

chitin, chitosan, biopolymers


Fermentation of shrimp biowaste was conducted with two Lactobacillus plantarum strains at pH=6.0 under various salt concentrations. The non-amylolytic strain L. plantarum 541 and amylolytic strain L. plantarum A6 showed to possess reasonable growth in biowaste with the addition of salt as high as 6 %. Fermentation carried with 10 % inoculum, 5 % glucose, with initial reduction of pH to 6.0 using acetic acid, showed that there was no spoilage of the biowaste. Growth trends in shrimp waste, in terms of CFU/mL developed on MRS-agar plates, changes in pH and lactic acid production (in g/L) were determined. The deproteination and demineralization efficiency with strain 541 was better than with strain A6. A maximum of 81.4 % demineralization and 59.8 % deproteination under 2 % salt conditions occurred with strain 541, whereas with strain A6, a demineralization of 65.5 % and deproteination of 52.2 % was observed.

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