Nutritional Requirements for the Mycelial Biomass and Exopolymer Production by Hericium erinaceus CZ-2

Daming Huang1, Fengjie Cui1*, Yin Li2, Zhicai Zhang1, Jiewen Zhao1, Xiaoming Han1, Xiang Xiao1, Jingya Qian1, Qifei Wu1 and Guoqiang Guan1

Institute of Bioengineering, School of Food and Biological Technology, Jiangsu University, 212013 Zhenjiang, PR China

2Department of Plant Science, North Dakota State University, 58105 Fargo, USA

Article history:

Received August 26, 2006
Accepted March 2, 2007

Key words:

Hericium erinaceus, submerged culture, medium optimization, mycelial biomass, exopolymer, orthogonal array design method


In this work, the effects of medium composition and fermentation parameters on the simultaneous production of mycelial biomass and exopolymer by medicinal mushroom Hericium erinaceus CZ-2 were investigated in shake flask cultures using one-factor-at-a-time method and orthogonal array design. Results showed that the most suitable carbon, nitrogen, mineral sources, and cofactors for the mycelial biomass and exopolymer production were: corn flour combined with 1 % glucose, yeast extract, KH2PO4 and corn steep liquor. The intuitive analysis of orthogonal array design results indicated that the effects of nutritional requirement on the mycelial growth of Hericium erinaceus CZ-2 were in regular sequence of corn flour combined with 1 % glucose > yeast extract > corn steep liquor > KH2PO4, and those on exopolymer production were in the order of corn flour combined with glucose > KH2PO4 > yeast extract > corn steep liquor. The maximal yield of mycelial biomass (16.07 g/L) was obtained when the composition of the culture medium was (in g/L): corn flour 30, glucose 10, yeast extract 3, KH2PO4 1, CaCO3 0.5, and 15 mL/L of corn steep liquor; while the maximal exopolymer yield (1.314 g/L) was achieved when the composition of medium was (in g/L): corn flour 30, glucose 10, yeast extract 5, KH2PO4 3, CaCO3 0.5, and 15 mL/L of corn steep liquor. In the 15-litre scale-up fermentation, the maximum mycelial biomass yield of 20.50 g/L was achieved using the optimized medium.


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