A New Oxidase-Peroxidase Kit for Ethanol Assays in Alcoholic Beverages
Mykhailo V. Gonchar1*, Mykola M. Maidan1, Halyna M. Pavlishko1 and Andrii A. Sibirny1,2
1Institute of Cell Biology, National Academy of Sciences, Drahomanov Street 14/16, Lviv 79005, Ukraine
2Rzeszów Pedagogical University, Rejtana Street 16 C, 35–310 Rzeszów, Poland
Article history:
Received May 2, 2000
Accepted October 26, 2000
Key words:
ethanol, enzymatic analysis, alcoholic beverages, alcohol oxidase, peroxidase, methylotrophic yeast
Summary:
The paper presents analytical parameters of an enzymatic method for alcohol assay in alcoholic beverages by the use of a new kit based on alcohol oxidase and peroxidase catalyzed reactions (AOP). Sensitivity, linearity, accuracy and reliability of the proposed method were tested. It has been shown that results of AOP-assays of alcohol in beers, wines, and strong drinks are in good correlation with those obtained by alcohol dehydrogenase (ADH) method (relations of ADH to AOP values are 0.9197±0.012; correlation coefficient R=0.9983; p<0.0001).
*Corresponding author: 
gonchar@biochem.Lviv.ua
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