A New Oxidase-Peroxidase Kit for Ethanol Assays in Alcoholic Beverages

Mykhailo V. Gonchar¹*, Mykola M. Maidan¹, Halyna M. Pavlishko¹ and Andrii A. Sibirny<sup>1,2 

1</sup>Institute of Cell Biology, National Academy of Sciences, Drahomanov Street 14/16, Lviv 79005, Ukraine
²Rzeszów Pedagogical University, Rejtana Street 16 C, 35–310 Rzeszów, Poland

Article history:
Received May 2, 2000
Accepted October 26, 2000

Key words:
ethanol, enzymatic analysis, alcoholic beverages, alcohol oxidase, peroxidase, methylotrophic yeast

Summary:
The paper presents analytical parameters of an enzymatic method for alcohol assay in alcoholic beverages by the use of a new kit based on alcohol oxidase and peroxidase catalyzed reactions (AOP). Sensitivity, linearity, accuracy and reliability of the proposed method were tested. It has been shown that results of AOP-assays of alcohol in beers, wines, and strong drinks are in good correlation with those obtained by alcohol dehydrogenase (ADH) method (relations of ADH to AOP values are 0.9197±
0.012; correlation coefficient R=0.9983; p<0.0001).

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