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Identification and Characterization of Yeast Isolates from Pharmaceutical Waste Water

Marjeta Recek, Neža Čadež and Peter Raspor*


University of Ljubljana, Biotechnical Faculty, Department Food Science and Technology, Jamnikarjeva 101, SI-1000 Ljubljana, Slovenia


Article history:

Received: March 30, 2001
Accepted: November 8, 2001

Key words:

COD, electrophoretic karyotyping, physiological test, Pichia anomala, rDNA –RFLP, waste water

Summary:

In order to develop an efficient an system for waste water pretreatment, the isolation of indigenous population of microorganisms from pharmaceutical waste water was done. We obtained pure cultures of 16 yeast isolates that differed slightly in colony morphology. Ten out of 16 isolates efficiently reduced COD in pharmaceutical waste water. Initial physiological characterization failed to match the 10 yeast isolates to either Pichia anomala or Pichia ciferrii. Restriction analysis of rDNA (rDNA-RFLP) using three different restriction enzymes: HaeIII, MspI and CfoI, showed identical patterns of the isolates and Pichia anomala type strain. Separation of chromosomal DNAs of yeast isolates by the pulsed field gel electrophoresis revealed that the 10 isolates could be grouped into 6 karyotypes. Growth characteristics of the 6 isolates with distinct karyotypes were then studied in batch cultivation in pharmaceutical waste water for 80 hours.



*Corresponding author:           peter.raspor@bf.uni-lj.si
                                               ++386 1 2574 092

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