Optimization of the Culture Medium for the Production of β-Galactosidase from Kluyveromyces marxianus CCT 7082

Ana Paula Manera, Joana da Costa Ores, Vanessa Amaral Ribeiro, Carlos André Veiga Burkert and Susana Juliano Kalil*

Department of Chemistry, Federal University Foundation of Rio Grande, P.O. Box 474, BR-96201-900 Rio Grande, RS, Brazil

Article history:

Received December 18, 2006
Accepted July 13, 2007

Key words:

β-galactosidase, Kluyveromyces marxianus, optimization, factorial design, response surface methodology


Seven strains of the genus Kluyveromyces were screened for β-galactosidase activity in a synthetic medium. Kluyveromyces marxianus CCT 7082 was selected as the best enzyme producer for optimization of the culture medium. A fractional factorial design 24–1 was used to determine the most relevant variables of the culture medium composition for the enzyme production. The parameters studied were the concentrations of lactose, yeast extract and (NH4)2SO4 as well as pH, and all were shown to have significant effects on the production of the enzyme. Based on the results from the first factorial design, lactose, yeast extract and (NH4)2SO4 concentrations were selected to be utilized in a 23 central composite rotatable design (CCRD). This led to a further optimization of the fermentation conditions to achieve higher enzyme activities, which reached 10.6 U/mL.


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