Influence of 2-deoxy-D-glucose on Cell Growth of the Yeast Saccharomyces cerevisiae

Marin Matošić, Srđan Novak and Vladimir Marić

Faculty of Food Technology and Biotechnology, University of Zagreb Pierottijeva 6, 10000 Zagreb, Croatia

Article history:
Received  March 28, 1996 
Accepted  June 18, 1996

Key words:
S. cerevisiae, cell growth, 2-dcoxy-D-glucose, inhibitory effects, glucose repression

Summary:
2-deoxy-D-glucose (2-DOG) causes two main effects in the yeast Saccharomyces cerevisiae: the first is glucose repression and the second is an inhibitory effect caused by a disturbance of cell metabolism. In this work we have studied both effects of 2-DOG on yeast metabolism and their influence on substrate uptake and growth of biomass. The effect of glucose repression on galactose grown cells was observed at 2-DOG concentration of 20 mg/L, and growth was stopped completely at the concentration of 50 mg/L. The inhibitory mode of action occurred at tlie concentrations higher than 300 mg/L.