Reversibility in Heterogeneous Flow Immunosensing and Related Techniques. A Brief Overview.
Miguel Angel González-Martínez1, Rosa Puchades1*, Angel Maquieira2
1Departarnento de Químíca, Universidad Politécnica de Valencia, Camino de Vera, 46022, Valencia, SPAIN
2Department of Entomology University of California, Davis, CA 95616, USA
Article history:
Received August 3, 2010
Accepted February 3, 2011
Key words:
immunoassay, reversibility, antigen/antibody interaction, immunoaffinty, immunochromatography
Summary:
In the development of many immunochemical techniques the reuse of the immunosorbent is one of the problems to be taken into account. The choice of an adequate desorbenl agent which accomplishes an effective desorp-lion without influencing the immunosorbent activity is the key to solving it. This is a review paper of the methods dealing with the problem of reversibility in immunochemical interactions and how the immunosurface life is modified, especially in fields as heterogeneous as flow immunoassays and immunoaffinity extraction and chromatography.