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The Effect of Autochthonous Starter Culture, Sugars and 
Temperature on the Fermentation of Slavonian Kulen

Krešimir Mastanjević1*, Dragan Kovačević1, Jadranka Frece2, Ksenija Markovand Jelka Pleadin3

1University of J. J. Strossmayer in Osijek, Faculty of Food Technology Osijek, Department of Food Technology, Kuhačeva 20, HR-31000 Osijek, Croatia
2University of Zagreb, Faculty of Food Technology and Biotechnology, Laboratory for General Microbiology and Food Microbiology, Pierottijeva 6, HR-10000 Zagreb, Croatia
3Croatian Veterinary Institute, Laboratory for Analytical Chemistry, Savska Cesta 143, HR-10000 Zagreb, Croatia


 


Article history:
Received  March 7, 2016
Accepted  October 12, 2016

 


Key words:
Slavonian kulen, autochthonous starter culture, sugars, fermentation temperature, physicochemical, microbiological and sensory properties


 

Summary:
In this study, the effect of an isolated and well-characterised autochthonous starter culture, glucose and maltodextrin (w=0.8 %) and temperatures of 12 and 20 °C on fermentation and quality of Slavonian kulen produced using the traditional technology and recipe were investigated. Physicochemical and microbiological analyses were carried out after 20 days of fermentation. Upon the completion of the production process (90 days), a sensory analysis was carried out. Furthermore, pH value was continuously measured throughout the twenty day fermentation period. The addition of an autochthonous starter culture and sugars and different fermentation temperatures significantly (p<0.05) affected the instrumental colour and texture parameters of the Slavonian kulen. The fermentation was most intense in the samples with added autochthonous starter culture and 0.8 % glucose, and fermented at 20 °C. Microbiological analysis showed that samples with added autochthonous starter culture and fermented at higher temperature contained a higher number of lactic acid bacteria and coagulase-negative staphylococci and were safe. Sensory evaluation confirmed the outcomes of physicochemical and microbiological analyses and showed differences among samples fermented at two different temperatures and with added glucose or maltodextrin and an autochthonous starter culture.



 


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