https://doi.org/10.17113/ftb.60.02.22.7285

Supplement

Applicability of Saccharomyces cerevisiae Strains for the Production of Fruit Wines Using Cocoa Honey Complemented with Cocoa Pulp 

Bárbara Teodora Andrade Koelher¹, Soraya Maria Moreira de Souza², Andréa Miura da Costa¹ and Elizama Aguiar-Oliveira^1*

¹State University of Santa Cruz (UESC), Rodovia Jorge Amado, km 16, Salobrinho, 45.662-900 Ilhéus, Bahia, Brazil

²Executive Commission for Cocoa Cultivation Planning (CEPLAC), Rodovia Jorge Amado, km 22, Primavera, 45.600-970 Ilhéus, Bahia, Brazil

Article history:

Received: 19 April 2021

Accepted: 20 December 2021

Keywords:

cocoa honey fermentation; cocoa pulp fermentation; Plackett-Burman design; S. cerevisiae L63; sweet wine production

Summary:

Research background. Cocoa honey and cocoa pulp are both highly appreciated fruit pulp, but until now, cocoa honey has been less processed than cocoa pulp. In this work, we investigate the applicability of Saccharomyces cerevisiae strains to ferment cocoa honey complemented with cocoa pulp to obtain fruit wines and improve cocoa honey commercialization.

Experimental approach. The strain, previously isolated from cachaçaria distilleries in Brazil, was selected based on its fermentation performance. The following conditions for fermentation with S. cerevisiae L63 were then studied: volume fraction of cocoa honey (φ_CH) complemented with cocoa pulp, sucrose addition (γ_suc), temperature (t) and inoculum size (N_o). The best conditions were applied in order to obtain fermentation profiles.

Results and conclusions. S. cerevisiae L63 (N_o=10⁷–10⁸ cell/mL) is capable of fermenting φ_CH=90 and 80 % for 24 or 48 h with γ_suc=50 and 100 g/L at t=28–30 °C resulting in wines with ethanol volume fractions from 8 to 14 %. Additionally, the wine produced from φ_CH=90 % had lower residual sugar concentration (<35 g/L) than the wine produced from φ_CH=80 % (~79 g/L) which could be classified as a sweet wine. In general, S. cerevisiae L63 resulted in a similar fermentation performance as a commercial strain tested, indicating its potential for fruit pulp fermentation.

Novelty and scientific contribution. Saccharomyyces cerevisiae L63 can ferment cocoa honey complemented with cocoa pulp to produce fruit wines with good commercial potential, which may also benefit small cocoa producers by presenting a product with greater added value.