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Immobilization of Providencia stuartii Cells in Pumice Stone and Its Application for N-Acetylglucosamine Production

Yuniwaty Halim1*orcid tiny, Devianita Devianita1, Hardoko Hardoko1,2orcid tiny, Ratna Handayani1orcid tiny and Lucia C. Soedirga1orcid tiny

1Food Technology Department, Universitas Pelita Harapan, Jl. M.H. Thamrin Boulevard, Lippo Karawaci, Tangerang 15811, Indonesia

2Faculty of Fisheries and Marine Sciences, Brawijaya University, Jl. Veteran No. 1, Malang 65113, Indonesia

Article history:

Received: 1 October 2020

Accepted: 12 November 2021

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cell immobilization; chitin degradation; N-acetylglucosamine production; Providencia stuartii; pumice stone; repeated fermentation


Research backgroundShrimp shells contain chitin that can be further processed into N-acetylglucosamine, which has been extensively used to treat joint damage. Providencia stuartii has a strong chitinolytic activity and may be utilized in the form of immobilized cells in repeated fermentation. Pumice is a porous and rigid stone that offers superior mechanical strength, making it suitable for immobilization.

Experimental approachIn the research submerged fermentation with different pumice stone sizes and pumice stone/growth medium ratios (m/V) was carried out for 4 days at 37 °C and pH=7.0. The optimum pumice stone size and pumice stone/growth medium ratio (m/V) were used to determine the optimum fermentation cycle for the production of N-acetylglucosamine using immobilized P. stuartii.

Results and conclusionsPumice stones of 1.0 cm×1.0 cm×1.0 cm and pumice stone/growth medium ratio of 1:5 were found to be the optimum conditions for successful immobilization of (90.0±1.6) % cells and production of (331.4±7.3) g/L N-acetylglucosamine. The highest N-acetylglucosamine concentration of (323.0±2.5) g/L was obtained in the first fermentation cycle, which then decreased and remained stable throughout the last three cycles.

Novelty and scientific contributionP. stuartii, a strong chitinolytic bacterium previously isolated from rotten shrimp shells, was used for the first time in immobilized form to produce N-acetylglucosamine. The findings in this research showed the potential use of P. stuartii cells immobilized in pumice stone for continuous production of N-acetylglucosamine in repeated fermentation.

*Corresponding author:

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