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Optimization, Purification and Characterization of Polygalacturonase from Mango Peel Waste Produced by Aspergillus foetidus

Sudheer Kumar Yannam1*, Prakasham Reddi Shetty2 and Vijaya Sarathi Reddy Obulum3


1
Centre for Structural and Functional Genomics, Concordia University, 7141 Sherbrooke West Montreal-H4B2E6, Canada
2Bioengineering and Environmental Centre, Indian Institute of Chemical Technology, Hyderabad-500007, India
3Department of Biochemistry, Sri Venkateswara University, Tirupati-517502, India

Article history
:

Received July 18, 2013
Accepted May 15, 2014

Key words
:

mango peel, Aspergillus foetidus, submerged fermentation, polygalacturonase, response surface methodology

Summary:

Pectin-rich mango peel from industrial waste was used as a substrate for the production and characterization of novel polygalactauronase enzyme. Its production was optimized with five important factors; KH2PO4, pH, peptone, MgSO4 and urea by employing response surface methodology using submerged fermentation with Aspergillus foetidus. Total of 50 experimental runs were carried out and the predicted values for optimization were in good agreement with experimental data. The results showed that a satisfactory production of polygalacturonase from the mango peel could be achieved, reaching up to 36.5 U/mL under optimized medium conditions of pH=5.8 and (in %, by mass per volume): KH2PO4 0.22, peptone 0.5, MgSO4 0.02 and urea 0.2 %. The polygalacturonase was partially purified to 3.4-fold and the molecular mass was found to be 34 kDa. The optimum pH and temperature for polygalacturonase activity were 5 and 55 °C, respectively.



*Corresponding author:        sudheerbiochem@gmail.com
                                         

                       

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