Continuous Enzymatic Prehydrolysis Treatment of High-Fat Wastewater

Rodrigo Augusto Franco de Oliveira Zawadzki1, Marcelo Real Prado2, David Alexander Mitchell3 and Nadia Krieger1*

1Department of Chemistry, Federal University of Paraná, Centro Politécnico, P.O. Box 19081, Curitiba 81531-980, Paraná, Brazil

2Department of Chemistry and Biology, Federal Technological University of Paraná, Av. Sete de Setembro, 3165, Curitiba 80230-901, Paraná, Brazil
3Department of Biochemistry and Molecular Biology, Federal University of Paraná, Centro Politécnico, P.O. Box 19046, Curitiba 81531-980, Paraná, Brazil

Article history:

Received May 13, 2012
Accepted March 8, 2013

Key words:

enzymatic hydrolysis, lipases, Rhizopus microsporus, high-fat wastewater, packed- -bed bioreactor


A lipolytic fermented solid was produced by solid-state fermentation of Rhizopus microsporus CPQBA 312-07 DRM on a mixture of sugarcane bagasse and sunflower seed meal and used, in a packed-bed bioreactor, to pretreat a high-fat wastewater from a meat and sausage processing factory located in São José dos Pinhais, State of Paraná, Brazil. With a hydraulic residence time of 24 h, this pretreatment not only reduced the wastewater’s oil and grease content by up to 96 %, but also increased its 5-day biochemical oxygen demand to chemical oxygen demand (BOD5/COD) ratio. This ratio was only 0.19 in the raw wastewater, indicating poor biodegradability, but increased to 0.55 in the pretreated wastewater, indicating that it had a sufficiently high biodegradability to be sent to a traditional anaerobic digestion or activated sludge process. After 96 days of operation of the packed bed, a microbiological analysis showed that R. microsporus was still present and viable in the fermented solid. Our work shows that a continuous packed-bed bioreactor containing fermented solid produced by R. microsporus has good potential for the treatment of high-fat wastewater.

*Corresponding author:
                                               ++55 41 3361 3470
                                               ++55 41 3361 3006

Search FTB

Follow us

 facebook 1 twitter bird_icon LI In Bug


Environmental Policy

sdg publishers compact 4 300x300

QR Code


We use cookies to improve our website and your experience when using it. Cookies used for the essential operation of the site have already been set. To find out more about the cookies we use and how to delete them, see our privacy policy.

I accept cookies from this site.

EU Cookie Directive Module Information