getpdf

Molecular Stability of Brain Plasmalogens in a Fat Free Diet

Jasna Dovhanj1, Ivančica Delaš2, Fritz Paltauf3 and Milivoj Popović2*


1
School of Medicine, University »Josip Juraj Strossmayer«, Osijek, Department of Chemistry and Biochemistry, Huttlerova 4, HR – 31 000 Osijek, Croatia

2School of Medicine, University of Zagreb, Department of Chemistry and Biochemistry, Šalata 3, HR – 10 000 Zagreb, Croatia
3Institut für Biochemie und Lebensmittelchemie der Technischen Universität, Petersgasse 12, A-8010 Graz, Austria

Article history:

Received February 24, 2000
Accepted May 17, 2000

Key words:

fat-free diet, plasmalogens, brain, rat

Summary:

Fat free diet (FFD) affects specifically structures of particular lipid molecules. The effect of short-term (two-week) diet on rat brain plasmalogens is reported in this paper. Two groups of eight animals (Wistar strain, average body weight 180 g) were on two different diets: the control diet (CD) was actually a standard rat chaw, while the fat free diet (FFD) was prepared in our own laboratory. Both diets were isoenergetic (CD 15.74 kJ; FFD 15.10 kJ). The average body weight gain was 4 %. For CD the mass of brain was 692.7 and for FFD 891.3 mg. Total phosphate/protein ratio (TP/P) of the brain tissue was 48.7/100 in CD and 33.2/100 in FFD, indicating the inhibited biosynthesis of phospholipids. The majority of plasmalogens in brain is associated with the phosphatidylethanolamine (PE) lipids. Plasmenyl-phosphatydilethanolamine (Pla-PE) comprised 58.3 % in CD and 59.7 % in FFD of total PE. Following effects of FFD on Pla-PE fatty acid composition were noticed: (i) increased content of palmitoleic acid (+3.45 %), (ii) increased content of stearic (+8.22 %) with decreased content of oleic acid (–6.02 %), (iii) decreased content of essential fatty acids (–12.55 %) and (iv) decreased content of polyunsaturated fatty acids followed by a simultaneous increase of eicosatrienoic acid content (+6.21 %) and a decrease of docosahexaenoic acid content (–8.47 %). The overall effect of FFD caused a moderate saturation of Pla-PE fatty acids (Fu/–7.39). The analysis of the aldehydogenic moiety of the Pla-PE resulted in: (i) no essential changes in the amount of total aldehydes of the PE lipids (CD 58.3 %, FFD 59.7 %); (ii) the alkenyl chain was composed of C 16, C 18 and C 20 homologues, while C 18 and C 20 homologues comprised almost 70 % of total aldehydes, (iii) no essential changes neither of the total amount of C 18 homologue (CD 59.4 %, FFD 64.1 %), nor of the ratio of cis-trans C 18 isomers were found, and finally (iv) the amount of C 20 homologue remained almost unchanged (CD 4.05 %, FFD 4.23 %). All of the represented data indicate very slight modulations of the Pla-PE molecule affected by a FFD and support the already recognized idea that plasmalogens are one of the most important stabilizers of the structure of biological membranes.



*Corresponding author:          idelas@mamef.mef.hr
                                               ++385 (0)1 4566 753
                                               +385 (0)1 4590 206

Search FTB


Follow us


 facebook 1 twitter bird_icon LI In Bug

 

QR Code


qrcode

We use cookies to improve our website and your experience when using it. Cookies used for the essential operation of the site have already been set. To find out more about the cookies we use and how to delete them, see our privacy policy.

I accept cookies from this site.

EU Cookie Directive Module Information