https://doi.org/10.17113/ftb.57.02.19.6142    


Influence of Dehydrated Wheat/Rice Cereal Matrices on Probiotic Activity of Bifidobacterium animalis ssp. lactis BB-12^®§ 


Andreja Leboš Pavunc¹, Lenkica Penava², Jasmina Ranilović³, Jasna Novak¹, Martina Banić¹, Katarina Butorac¹, Emilija Petrović³, Vesna Mihaljević-Herman³, Krešo Bendelja⁴, Ana Savić Mlakar⁴, Ksenija Durgo⁵, Blaženka Kos¹* and Jagoda Šušković<sup>1  


1</sup>University of Zagreb, Faculty of Food Technology and Biotechnology, Laboratory of Antibiotic, Enzyme, Probiotic and Starter Culture Technologies, Pierottijeva 6, 10000 Zagreb, Croatia 
²Belupo, Pharmaceuticals & Cosmetics Inc., Nutraceuticals, Business Development and Registration, I. Savica 36, 10000 Zagreb, Croatia
³Podravka, Research and Development, Ante Starčevića 32, 48000 Koprivnica, Croatia
⁴University of Zagreb, Centre for Research and Knowledge Transfer in Biotechnology, Rockefellerova 10, 10000 Zagreb, Croatia
⁵University of Zagreb, Faculty of Food Technology and Biotechnology, Laboratory for Biology and Microbial Genetics, Pierottijeva 6, 10000 Zagreb, Croatia 



Article history:
Received: 21 November 2018
Accepted: 18 June 2019
 



Key words: 
Bifidobacterium, probiotics, dehydrated wheat/rice cereal products



Summary: 
Three novel dehydrated wheat/rice cereal functional products with an addition of well documented probiotic Bifidobacterium animalis ssp. lactis BB-12^® (BB-12^®) were developed in Podravka factory for the infants older than 4 months: instant rice cereal, instant rice cereal with fruits and instant wheat cereal with vanilla. Notably, the number of viable BB-12^® cells in each of the novel products was higher than the required minimal number of probiotic cells per gram of product (106 CFU/g) during the storage period of 106 weeks. Therefore, BB-12^® strain recovery and genome stability were evaluated by strain-specific polimerase chain reaction and amplified fragment length polymorphism fingerprinting analysis. Further aim was to evaluate the influence of these three different cereal food matrices on specific probiotic properties of BB-12^® strain in vitro. Applied food matrices positively influenced the survival in the simulated conditions of the gastrointestinal tract and antagonistic activity against undesirable microorganisms, while no influence on auto- and coaggregation ability of B. animalis ssp. lactis BB-12^® was observed. Adhesion to extracellular matrix proteins and intestinal epithelial Caco-2 cells together with antibacterial activity emphasized competitive pathogen exclusion from Caco-2 cells by probiotic strain BB-12^®. 





*Corresponding author:    +38514605291 
                                             +38514836424  
                                              bkos@pbf.hr

^§Paper was presented at the 9^th International Congress of Food Technologists, Biotechnologists and Nutritionists, 3-5 October 2018, Zagreb, Croatia