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Influence of Dehydrated Wheat/Rice Cereal Matrices on Probiotic Activity of Bifidobacterium animalis ssp. lactis BB-12®§ 

Andreja Leboš Pavunc1orcid tiny, Lenkica Penava2orcid tiny, Jasmina Ranilović3orcid tinyJasna Novak1orcid tiny, Martina Banić1orcid tiny, Katarina Butorac1orcid tiny, Emilija Petrović3orcid tinyVesna Mihaljević-Herman3orcid tiny, Krešo Bendelja4orcid tiny, Ana Savić Mlakar4orcid tiny, Ksenija Durgo5orcid tiny, Blaženka Kos1*orcid tiny and Jagoda Šušković1orcid tiny  

1University of Zagreb, Faculty of Food Technology and Biotechnology, Laboratory of Antibiotic, Enzyme, Probiotic and Starter Culture Technologies, Pierottijeva 6, 10000 Zagreb, Croatia 
2Belupo, Pharmaceuticals & Cosmetics Inc., Nutraceuticals, Business Development and Registration, I. Savica 36, 10000 Zagreb, Croatia
3Podravka, Research and Development, Ante Starčevića 32, 48000 Koprivnica, Croatia
4University of Zagreb, Centre for Research and Knowledge Transfer in Biotechnology, Rockefellerova 10, 10000 Zagreb, Croatia
5University of Zagreb, Faculty of Food Technology and Biotechnology, Laboratory for Biology and Microbial Genetics, Pierottijeva 6, 10000 Zagreb, Croatia 

Article history:
Received: 21 November 2018
Accepted: 18 June 2019

Key words
Bifidobacterium, probiotics, dehydrated wheat/rice cereal products

Three novel dehydrated wheat/rice cereal functional products with an addition of well documented probiotic Bifidobacterium animalis ssp. lactis BB-12® (BB-12®) were developed in Podravka factory for the infants older than 4 months: instant rice cereal, instant rice cereal with fruits and instant wheat cereal with vanilla. Notably, the number of viable BB-12® cells in each of the novel products was higher than the required minimal number of probiotic cells per gram of product (106 CFU/g) during the storage period of 106 weeks. Therefore, BB-12® strain recovery and genome stability were evaluated by strain-specific polimerase chain reaction and amplified fragment length polymorphism fingerprinting analysis. Further aim was to evaluate the influence of these three different cereal food matrices on specific probiotic properties of BB-12® strain in vitro. Applied food matrices positively influenced the survival in the simulated conditions of the gastrointestinal tract and antagonistic activity against undesirable microorganisms, while no influence on auto- and coaggregation ability of B. animalis ssp. lactis BB-12® was observed. Adhesion to extracellular matrix proteins and intestinal epithelial Caco-2 cells together with antibacterial activity emphasized competitive pathogen exclusion from Caco-2 cells by probiotic strain BB-12®

*Corresponding author:  tel3  +38514605291 
                                           fax2  +38514836424  


§Paper was presented at the 9th International Congress of Food Technologists, Biotechnologists and Nutritionists, 3-5 October 2018, Zagreb, Croatia


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